Nonapeptide-1 (200MG)

What Is Nonapeptide-1 and Why Buy It for Research?

Buy Nonapeptide-1 in Switzerland for research into melanogenesis, melanocortin receptor signaling, and hyperpigmentation disorders. Specifically, Nonapeptide-1 (also known as Melanostatine-5) is a synthetic nonapeptide composed of nine amino acids with the sequence Met-Pro-D-Phe-Arg-D-Trp-Phe-Lys-Pro-Val-NH₂ (one-letter code: MPFRWFKPV-NH₂). The peptide incorporates D-amino acid residues at positions 3 (D-Phe) and 5 (D-Trp), a structural feature critical for its antagonistic activity at the melanocortin-1 receptor (MC1R).

Researchers discovered Nonapeptide-1 through screening a multi-use peptide library based on the α-MSH (5-13) sequence. Structural analysis reveals that residues at positions 5-6, 7-9, and 10 are critical for its antagonistic activity, with D-Trp5 and Phe6 playing pivotal roles in receptor inactivation. The peptide has a molecular weight of 1,206.5 g/mol, the molecular formula C₆₁H₈₇N₁₅O₉S, and the CAS number 158563-45-2.

In scientific research, Nonapeptide-1 serves as a molecular tool for studying MC1R-mediated signaling pathways, melanogenesis regulation, and skin pigmentation mechanisms. Furthermore, Core Peptides supplies Nonapeptide-1 as a lyophilized powder in a 200mg format exclusively for laboratory use. Therefore, researchers seeking to source this compound for their work will find it readily available when they buy Nonapeptide-1 in Switzerland.

Nonapeptide-1 Research Overview: Buy Nonapeptide-1 in Switzerland for MC1R Studies

Centrally, research into Nonapeptide-1 has focused on its function as a potent and selective antagonist of the melanocortin-1 receptor (MC1R). MC1R is a G-protein coupled receptor expressed on the surface of melanocytes that plays a central role in regulating melanin synthesis and skin pigmentation. The endogenous ligand α-melanocyte-stimulating hormone (α-MSH) binds to MC1R and activates a signaling cascade that ultimately leads to melanin production.

Nonapeptide-1 acts as a competitive antagonist of α-MSH, binding to MC1R without activating the downstream signaling cascade. This competitive inhibition prevents α-MSH-induced activation of adenylyl cyclase, thereby reducing intracellular cyclic AMP (cAMP) levels. The peptide exhibits a binding affinity (Ki) of 40 nM for MC1R in COS-1 cells expressing human receptors. Nonapeptide-1 is selective for MC1R over MC3R, MC4R, and MC5R, with Ki values of 470 nM, 1,340 nM, and 2,400 nM, respectively.

By blocking MC1R signaling, Nonapeptide-1 leads to significant downregulation of key melanogenic enzymes, including tyrosinase, TRP-1, and TRP-2, as well as the master transcription factor MITF. The peptide effectively inhibits α-MSH-induced intracellular cAMP accumulation (IC50: 2.5 nM) and melanosome dispersion (IC50: 11 nM) in melanocytes. Studies demonstrate that Nonapeptide-1 reduces melanin synthesis by approximately 33% in melanocyte models. Accordingly, researchers who buy Nonapeptide-1 in Switzerland from Core Peptides can support their investigations into MC1R signaling, melanogenesis regulation, and pigmentation disorders.

How Does Nonapeptide-1 Work in Research?

Mechanistically, Nonapeptide-1’s proposed action involves functioning as a competitive antagonist of the melanocortin-1 receptor (MC1R) that blocks α-MSH-mediated signaling. The peptide operates through a well-characterized mechanism involving several key steps.

Competitive MC1R Antagonism

Nonapeptide-1 binds to MC1R with high affinity (Ki: 40 nM) and competes with the endogenous ligand α-MSH for receptor occupancy. The peptide’s D-amino acid residues at positions 3 (D-Phe) and 5 (D-Trp) are critical for its antagonistic properties, with D-Trp5 and Phe6 playing pivotal roles in receptor inactivation. Unlike agonists that activate MC1R signaling, Nonapeptide-1 binds to the receptor without triggering downstream activation, effectively acting as a “neutral” antagonist that prevents α-MSH from initiating the melanogenesis cascade.

cAMP Pathway Inhibition

By blocking α-MSH binding to MC1R, Nonapeptide-1 prevents the activation of adenylyl cyclase and the subsequent production of intracellular cyclic AMP (cAMP). The peptide inhibits α-MSH-induced cAMP accumulation with an IC50 of 2.5 nM. This reduction in cAMP levels prevents the activation of protein kinase A (PKA) and the downstream phosphorylation of the cAMP response element-binding protein (CREB).

Downregulation of Melanogenic Enzymes

By suppressing the cAMP/PKA/CREB signaling axis, Nonapeptide-1 reduces the expression of Microphthalmia-associated Transcription Factor (MITF), the master regulator of melanocyte differentiation and melanogenesis. This leads to decreased transcription of key melanogenic enzymes, including tyrosinase (TYR), tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2). Tyrosinase is the rate-limiting enzyme in the melanin synthesis pathway, and its reduced expression directly correlates with decreased melanin production.

Melanin Synthesis Inhibition

The net effect of MC1R antagonism and downstream signaling inhibition is a significant reduction in melanin synthesis. Studies have shown that Nonapeptide-1 reduces melanin content by approximately 33% in melanocyte models without affecting normal cellular functions. In human epidermal melanocytes, Nonapeptide-1 at 20 μM reverses UVA-induced melanin growth and suppresses basal melanin synthesis.

Why Is Nonapeptide-1 Studied in Switzerland?

Primarily, researchers study Nonapeptide-1 because it represents a well-characterized MC1R antagonist that enables investigation of melanogenesis, pigmentation regulation, and melanocortin receptor pharmacology. In fact, this compound offers a focused tool for studying the molecular mechanisms underlying skin pigmentation, hyperpigmentation disorders, and melanocyte biology.

Research has demonstrated Nonapeptide-1’s relevance across multiple domains. The peptide has been shown to inhibit melanin synthesis by approximately 33% in melanocyte models. Studies have demonstrated that Nonapeptide-1 effectively blocks α-MSH-induced intracellular cAMP accumulation and melanosome dispersion. Furthermore, the peptide downregulates the expression of MC1R, tyrosinase, TRP-1, TRP-2, and MITF in human epidermal melanocytes and HaCaT cells.

Additionally, Nonapeptide-1 has been investigated for its potential applications in hyperpigmentation disorders, including melasma, post-inflammatory hyperpigmentation, and UV-induced pigmentation. The peptide’s selectivity for MC1R over other melanocortin receptors makes it a valuable tool for studying MC1R-specific signaling pathways. Ultimately, researchers who buy Nonapeptide-1 in Switzerland gain access to a comprehensive research tool for investigating melanogenesis, pigmentation regulation, and MC1R pharmacology.

Nonapeptide-1 Research Areas in Laboratory Studies

Research Area	Focus	Evidence Level
Melanogenesis Research	Melanin synthesis inhibition, tyrosinase downregulation, MITF modulation	Mechanistic / In Vitro
MC1R Receptor Pharmacology	Receptor binding, competitive antagonism, receptor selectivity	Mechanistic / In Vitro
Hyperpigmentation Disorders	Melasma, post-inflammatory hyperpigmentation, UV-induced pigmentation	Preclinical
Skin Pigmentation Research	Melanocyte biology, pigmentation regulation, skin lightening mechanisms	Mechanistic / In Vitro
Melanocortin Receptor Signaling	cAMP pathway inhibition, PKA/CREB/MITF axis, receptor selectivity	Mechanistic
Dermatological Research	Skin pigmentation disorders, cosmetic dermatology, photoprotection	Preclinical

Nonapeptide-1 Compared With Related Research Compounds

Compound	Research Focus	Mechanism / Target	Key Difference
Nonapeptide-1 (Melanostatine-5)	MC1R antagonism, melanogenesis inhibition, pigmentation research	Competitive MC1R antagonist (Ki: 40 nM)	9-amino acid peptide with D-amino acids; selective MC1R antagonist
Decapeptide-12	Tyrosinase inhibition, melanogenesis research	Competitive tyrosinase inhibitor (IC50: 40 µM)	Different mechanism; directly inhibits tyrosinase enzyme
α-MSH	Native melanocortin signaling	MC1R agonist	Endogenous ligand; activates melanogenesis; opposite effect
Kojic Acid	Hyperpigmentation, skin lightening	Tyrosinase inhibitor (chelates copper)	Small molecule; different mechanism; less potent

Comparatively, Nonapeptide-1 differs from other depigmenting agents in its receptor-targeted mechanism. On the one hand, Nonapeptide-1 acts upstream in the melanogenesis pathway by blocking MC1R receptor activation, preventing the entire signaling cascade from being initiated. On the other hand, tyrosinase inhibitors such as Decapeptide-12 and kojic acid act downstream by directly inhibiting the tyrosinase enzyme. In contrast, α-MSH is the endogenous agonist that activates MC1R and stimulates melanogenesis, representing the opposite pharmacological effect.

Research Applications for Nonapeptide-1 in Switzerland

Scientists have investigated Nonapeptide-1 across several research domains:

• Melanogenesis Research: Researchers use Nonapeptide-1 to study melanin synthesis inhibition, tyrosinase downregulation, and MITF modulation in melanocyte models.
• MC1R Receptor Pharmacology: The peptide serves as a tool for investigating receptor binding, competitive antagonism, and MC1R selectivity over other melanocortin receptors.
• Hyperpigmentation Studies: Nonapeptide-1 has been studied for its potential applications in melasma, post-inflammatory hyperpigmentation, and UV-induced pigmentation disorders.
• Skin Pigmentation Research: The peptide is employed to investigate melanocyte biology, pigmentation regulation, and skin lightening mechanisms.
• Dermatological Research: Studies have examined Nonapeptide-1’s potential in cosmetic dermatology and photoprotection research.
• Melanocortin Receptor Signaling: The compound is used to study cAMP pathway inhibition, PKA/CREB/MITF axis regulation, and receptor selectivity.

Evidently, researchers who buy Nonapeptide-1 in Switzerland gain a valuable addition to any laboratory toolkit focused on melanogenesis, pigmentation research, and MC1R pharmacology.

Nonapeptide-1 Product Specifications

Specification	Details
Product Name	Nonapeptide-1 (Melanostatine-5)
Alternative Names	Melanostatine-5, Met-Pro-D-Phe-Arg-D-Trp-Phe-Lys-Pro-Val-NH₂, MPFRWFKPV-NH₂
Amount	200mg
Form	Lyophilized powder
Purity	Not verified (≥95-98% HPLC purity reported by some suppliers)
Amino Acid Sequence	H-Met-Pro-D-Phe-Arg-D-Trp-Phe-Lys-Pro-Val-NH₂
Sequence (1-letter)	MPFRWFKPV-NH₂ (f=D-Phe, w=D-Trp)
Peptide Length	9 amino acids (nonapeptide)
Molecular Formula	C₆₁H₈₇N₁₅O₉S
Molecular Weight	1,206.5 g/mol
CAS Number	158563-45-2
PubChem CID	10418849
MC1R Binding Affinity (Ki)	40 nM (human MC1R)
IC50 (cAMP inhibition)	2.5 nM
IC50 (Melanosome dispersion)	11 nM
Research Category	Skin, Hair & Beauty / Cellular Research & Signaling
Research Use	Laboratory research use only
CoA	Available upon request (not verified)
Storage	Lyophilized powder should be stored desiccated at -20°C or below, protected from light
Solubility	Soluble in DMSO and water

How to Evaluate Nonapeptide-1 Quality for Research

When sourcing Nonapeptide-1 peptide for laboratory research, researchers should consider several quality indicators:

• Identity Confirmation: Analytical techniques such as mass spectrometry should confirm the compound’s molecular weight (1,206.5 g/mol) and sequence (Met-Pro-D-Phe-Arg-D-Trp-Phe-Lys-Pro-Val-NH₂).
• Purity Assessment: High-performance liquid chromatography (HPLC) should verify purity, with ≥95% considered standard for research-grade material.
• D-Amino Acid Confirmation: Analytical methods should confirm the presence of D-amino acid residues at positions 3 (D-Phe) and 5 (D-Trp), as these are critical for biological activity.
• Batch Traceability: Lot numbers and batch information enable researchers to track specific production runs.
• Certificate of Analysis: A CoA should accompany the product, detailing analytical testing results including HPLC purity and identity confirmation.
• Labeling Accuracy: Proper labeling should clearly indicate the product name, amount (200mg), sequence, molecular formula, CAS number, and storage recommendations.
• Storage Conditions: Appropriate storage at -20°C or below in desiccated, dark conditions helps maintain peptide stability.

Understanding the Certificate of Analysis

Typically, a Certificate of Analysis (CoA) accompanies research peptides and provides information about the specific batch’s analytical testing. For example, a CoA may include product identity confirmation, purity percentage as determined by HPLC, batch or lot number for traceability, analytical methods used (e.g., mass spectrometry, HPLC), and a physical description of the product.

Nevertheless, it is important to note that a CoA does not establish safety, efficacy, therapeutic effectiveness, or suitability for human use. Instead, it serves as a quality control document that helps researchers verify the identity and purity of the peptide they are using in their laboratory investigations.

Storage and Stability of Nonapeptide-1

Core Peptides supplies Nonapeptide-1 as a lyophilized (freeze-dried) powder. To maintain stability and integrity:

• Store the lyophilized powder desiccated at -20°C or below for long-term storage
• Store at 4°C for short-term storage if frequent access is required
• Avoid repeated freeze-thaw cycles
• Weigh under dry conditions; the peptide is hygroscopic
• Reconstitute only immediately before use with appropriate sterile solvents (sterile water, DMSO, or buffered aqueous solutions)
• Inject solvent slowly against the inner wall of the vial; avoid vigorous agitation during dissolution
• Filter sterilize if required (e.g., 0.22 µm) immediately prior to use
• Aliquot and store reconstituted solutions at -20°C or below to minimize degradation
• Protect from light and moisture; prolonged exposure to light, elevated temperature, or aqueous environments may reduce stability

Frequently Asked Questions About Buying Nonapeptide-1 in Switzerland

What is Nonapeptide-1?

Specifically, Nonapeptide-1 (Melanostatine-5) is a synthetic nonapeptide with the sequence Met-Pro-D-Phe-Arg-D-Trp-Phe-Lys-Pro-Val-NH₂. It is a potent and selective antagonist of the melanocortin-1 receptor (MC1R) that competitively blocks α-MSH binding and inhibits melanin synthesis.

What is Nonapeptide-1 researched for?

Primarily, researchers study Nonapeptide-1 for its role as an MC1R antagonist in melanogenesis, skin pigmentation, and hyperpigmentation research. Studies have investigated its effects on melanin synthesis inhibition, tyrosinase downregulation, and MC1R receptor pharmacology.

How does Nonapeptide-1 work?

Mechanistically, Nonapeptide-1 functions as a competitive antagonist of MC1R, binding to the receptor without activating downstream signaling. This prevents α-MSH-induced activation of adenylyl cyclase, reduces intracellular cAMP levels, and downregulates melanogenic enzymes including tyrosinase, TRP-1, and TRP-2.

What is the amino acid sequence of Nonapeptide-1?

The amino acid sequence is H-Met-Pro-D-Phe-Arg-D-Trp-Phe-Lys-Pro-Val-NH₂ (one-letter code: MPFRWFKPV-NH₂, with f=D-Phe and w=D-Trp).

What is the molecular weight of Nonapeptide-1?

The molecular weight is 1,206.5 g/mol.

What is the molecular formula of Nonapeptide-1?

The molecular formula is C₆₁H₈₇N₁₅O₉S.

What is the CAS number of Nonapeptide-1?

The CAS number is 158563-45-2.

What is the binding affinity of Nonapeptide-1 for MC1R?

Nonapeptide-1 binds to human MC1R with a Ki of 40 nM. It is selective for MC1R over MC3R (Ki: 470 nM), MC4R (Ki: 1,340 nM), and MC5R (Ki: 2,400 nM).

Is Nonapeptide-1 synthetic?

Yes, Nonapeptide-1 is a synthetic peptide produced through solid-phase peptide synthesis with D-amino acid residues at positions 3 and 5.

What research exists on Nonapeptide-1?

Research on Nonapeptide-1 includes studies demonstrating MC1R binding affinity (Ki: 40 nM), inhibition of α-MSH-induced cAMP accumulation (IC50: 2.5 nM), inhibition of melanosome dispersion (IC50: 11 nM), and approximately 33% reduction in melanin synthesis in melanocyte models.

Is Nonapeptide-1 approved as a medicine?

No. Regulatory authorities have not approved Nonapeptide-1 as a medicine, pharmaceutical, or therapeutic agent. It is a research tool for laboratory use only.

Is Nonapeptide-1 intended for human use?

No. Nonapeptide-1 is intended for laboratory research use only and is not for human consumption, injection, or therapeutic application.

Where can I buy Nonapeptide-1 in Switzerland?

You can buy Nonapeptide-1 in Switzerland from Core Peptides, a trusted research peptide supplier serving the Swiss scientific community.

How should researchers evaluate Nonapeptide-1 quality?

Researchers should look for identity confirmation via mass spectrometry, purity data from HPLC, D-amino acid confirmation, batch traceability, and a Certificate of Analysis.

Related Research Compounds

Researchers interested in Nonapeptide-1 may also find the following related compounds relevant to their work:

• Decapeptide-12 — A tyrosinase inhibitor peptide researched for melanogenesis and hyperpigmentation studies.
• α-MSH — The endogenous MC1R agonist that stimulates melanogenesis, used for comparative receptor pharmacology studies.
• GHK-Cu — A copper-binding peptide researched for skin regeneration, wound healing, and anti-aging applications.
• BPC-157 — A peptide researched for tissue healing, gastrointestinal protection, and angiogenesis.

Explore Skin, Hair & Beauty

Nonapeptide-1 belongs to the Skin, Hair & Beauty category of research compounds. Skin, Hair & Beauty products are studied for their potential roles in dermatological research, pigmentation modulation, and skin health. Generally, researchers who buy Nonapeptide-1 in Switzerland often pair it with related compounds in this category to explore complementary pathways in melanogenesis and skin research.

Explore Cellular Research & Signaling

Researchers studying melanocortin receptor signaling, cAMP pathways, and cellular signaling may also find relevant compounds in the Cellular Research & Signaling category. Nonapeptide-1’s interactions with MC1R and the cAMP/PKA/CREB/MITF axis make it a valuable tool for investigating fundamental cellular signaling and receptor pharmacology.

Further Research

For researchers seeking to deepen their understanding of Nonapeptide-1 and its research applications, the following areas may warrant further investigation:

• The relationship between Nonapeptide-1-mediated MC1R antagonism and specific downstream effects on melanogenesis pathways
• Comparative studies between Nonapeptide-1 and other MC1R antagonists in various pigmentation and melanocyte models
• The potential role of Nonapeptide-1 in modulating melanocortin receptor signaling beyond MC1R
• Mechanistic studies on Nonapeptide-1’s interaction with MC1R and its functional consequences for cAMP signaling and melanin synthesis
• Exploration of Nonapeptide-1’s effects on different cell types and tissue systems beyond melanocytes

Research Disclaimer

Nonapeptide-1 is a research peptide supplied for laboratory research use only. Researchers must not use it for human consumption, injection, or any form of therapeutic application. Moreover, this product is not a medicine, pharmaceutical, or medical device. Furthermore, Swissmedic, the FDA, EMA, or any other regulatory authority have not approved it for human use. Researchers should consult Swissmedic for applicable regulations and guidance on research peptide handling in Switzerland.

Generally, the information provided on this page is for educational and scientific reference purposes only. Therefore, it does not constitute medical advice, treatment recommendations, or dosing instructions. Ultimately, researchers bear the responsibility for ensuring that their use of this product complies with all applicable laws, regulations, and institutional guidelines.

Finally, all research involving peptides should be conducted by qualified personnel in appropriate laboratory settings with proper safety protocols.