Syn-AKE (200MG)

What Is Syn-AKE?

Syn-AKE, also known as Dipeptide Diaminobutyroyl Benzylamide Diacetate, is a synthetic dipeptide with the amino acid sequence β-Ala-Pro-Dab-NHBzl. It belongs to a class of biomimetic peptides designed to emulate the neuromuscular blocking activity of Waglerin-1, a peptide found in the venom of the Temple Viper (Tropidolaemus wagleri). For those looking to buy Syn-AKE in Switzerland, Core Peptides offers high-quality research-grade material for laboratory applications.

As a dipeptide a peptide composed of two amino acids with a modified C-terminal benzylamide Syn-AKE features a unique structure that allows it to interact with nicotinic acetylcholine receptors (nAChR) at the neuromuscular junction. The peptide is commonly supplied as the diacetate salt with the molecular formula C23H37N5O7 and a molecular weight of approximately 495.58 g/mol. Its INCI name is Dipeptide Diaminobutyroyl Benzylamide Diacetate, and it is identified by CAS number 823202-99-9.

Syn-AKE Research Overview

Syn-AKE has attracted research interest as a tool for investigating neuromuscular junction signaling, nicotinic acetylcholine receptor pharmacology, and synaptic transmission. The peptide’s design a synthetic mimic of the active region of Waglerin-1 enables it to reversibly antagonize muscular nicotinic acetylcholine receptors (mnAChR) at the postsynaptic membrane.

Research interest in Syn-AKE centers on its ability to modulate neuromuscular transmission through competitive inhibition of acetylcholine binding. Specifically, by binding to the nAChR, Syn-AKE prevents acetylcholine from activating the receptor, thereby reducing muscle contraction. Consequently, this mechanism positions Syn-AKE as a valuable compound for studying receptor-ligand interactions, neuromuscular modulation, and synaptic transmission.

When researchers choose to buy Syn-AKE in Switzerland for laboratory studies, they gain access to a compound that may help elucidate mechanisms of nAChR antagonism, neuromuscular junction physiology, and receptor pharmacology at the molecular level.

How Does Syn-AKE Work?

Syn-AKE exerts its biological effects through reversible antagonism of muscular nicotinic acetylcholine receptors (mnAChR) at the postsynaptic membrane. The peptide’s mechanism involves several well-characterized steps.

Nicotinic Acetylcholine Receptor Binding

Syn-AKE acts as a reversible antagonist at the muscular nicotinic acetylcholine receptor (mnAChR). Research suggests that the peptide binds to the C-loop of the α1 subunit of the nAChR, thereby inhibiting acetylcholine from binding to the receptor. As the muscular nicotinic ACh receptors are reversibly blocked, the ion channel remains closed, preventing the depolarization that would normally trigger muscle contraction.

In previous studies, acetylcholine has been shown to bind to the C-loop of subunit α1, the allosteric site of the nAChR. Syn-AKE appears to compete for this binding site, thereby attenuating the nicotinic response.

Neuromuscular Junction Modulation

Syn-AKE is a postsynaptic peptide, meaning it acts on the receiving side of the neuromuscular junction. By occupying some of the receptors at the nerve-to-muscle junction, the peptide reduces the micro-movements that drive muscle contraction. In muscle cell contraction assays, Syn-AKE has been shown to reduce contraction frequency by up to 82% at optimal concentrations, demonstrating potent but reversible neuromuscular modulation.

Reversible Antagonism

Unlike botulinum toxin, which enzymatically cleaves SNARE proteins and produces irreversible effects, Syn-AKE acts as a reversible antagonist of the neuromuscular postsynaptic nicotinic acetylcholine receptor. This reversible mechanism allows for the study of receptor dynamics and the recovery of function following peptide administration.

Researchers who buy Syn-AKE in Switzerland for research purposes can investigate these mechanisms across diverse experimental models of neuromuscular transmission, receptor pharmacology, and synaptic signaling.

Why Is Syn-AKE Studied?

Researchers study Syn-AKE primarily for its potential relevance to nicotinic acetylcholine receptor pharmacology, neuromuscular junction physiology, and synaptic transmission. The peptide’s ability to reversibly antagonize nAChR makes it a valuable tool for understanding the molecular basis of neuromuscular signaling and receptor-ligand interactions.

Syn-AKE’s connection to the Waglerin-1 venom peptide has made it a focus of neuropharmacology and biomimetic research. For instance, the peptide represents a synthetic approach to studying the neuromuscular blocking activity of natural venom components without the toxicity of the natural compound. This enables researchers to investigate receptor binding kinetics and structure-activity relationships in a controlled laboratory setting.

Moreover, researchers study Syn-AKE in the context of receptor pharmacology and drug development. Head-to-head comparisons in in vitro assay systems suggest that Syn-AKE may achieve faster onset of neuromuscular modulation compared to other peptides. Therefore, this makes it a useful reference compound for investigating nAChR antagonists and their effects on synaptic transmission.

Researchers who buy Syn-AKE in Switzerland from Core Peptides can incorporate this peptide into their studies on nAChR pharmacology, neuromuscular junction signaling, and receptor-mediated modulation of muscle contraction.

Syn-AKE Research Areas

Research Area	Description
Nicotinic Acetylcholine Receptor Pharmacology	Investigating Syn-AKE’s binding affinity and antagonism of nAChR
Neuromuscular Junction Signaling	Studying Syn-AKE’s effects on neuromuscular transmission and muscle contraction
Synaptic Transmission	Examining Syn-AKE’s modulation of neurotransmitter release and receptor activation
Receptor-Ligand Interactions	Exploring the molecular basis of nAChR binding and antagonism
Reversible Neuromuscular Blockade	Preclinical research into reversible nAChR antagonism and receptor dynamics
Biomimetic Peptide Research	Investigating synthetic mimics of natural venom peptides
Muscle Contraction Modulation	Studying Syn-AKE’s effects on contraction frequency and amplitude
Receptor Binding Studies	Investigating the molecular interactions between Syn-AKE and nAChR subunits

Scientific Research on Syn-AKE

Researchers have conducted scientific studies on Syn-AKE in both in vitro and preclinical settings, deriving evidence from receptor-binding assays, muscle contraction assays, and molecular docking experiments. The peptide originates from research on the Temple Viper venom peptide Waglerin-1 and represents a synthetic biomimetic approach to studying nAChR antagonism.

In Vitro Evidence

In vitro studies using receptor-binding assays have demonstrated that Syn-AKE acts as a reversible antagonist of muscular nicotinic acetylcholine receptors (mnAChR). The peptide binds to the C-loop of the α1 subunit of the nAChR, inhibiting acetylcholine from binding to the receptor. Researchers have characterized this mechanism through electrophysiological and pharmacological studies.

Muscle cell contraction assays have shown that Syn-AKE reduces contraction frequency by up to 82% at optimal concentrations, demonstrating potent but reversible neuromuscular modulation. Furthermore, head-to-head comparisons in in vitro assay systems suggest that Syn-AKE may achieve faster onset of neuromuscular modulation compared to other peptides.

Structural and Synthetic Studies

Researchers have developed synthetic routes for Syn-AKE, including an optimized Hofmann-type rearrangement as a final step to build the target skeleton. The synthesis of H-β-Ala-Pro-DabNHBz diacetate (a muscular nicotinic acetylcholine receptor antagonist) has been delineated through this five-step synthetic pathway.

The peptide’s structure has been characterized with the sequence β-Ala-Pro-Dab-NHBzl and molecular formula C23H37N5O7. Research-grade material is typically supplied as a lyophilized powder with verified HPLC purity ≥98%.

Clinical Studies

Syn-AKE has been studied in clinical research for its effects on wrinkle formation and expression lines. For example, a 2021 double-blind, randomized clinical trial examined the effects of a peptide serum containing Syn-AKE on skin appearance. However, researchers should note that the peptide is supplied for laboratory research purposes only. Scientists who buy Syn-AKE in Switzerland should remain aware of these evidence considerations.

Syn-AKE Compared With Related Peptides

Peptide	Relationship to Syn-AKE	Key Differences
Waglerin-1	Natural venom peptide; Syn-AKE is a synthetic mimic	Waglerin-1 is a 22-amino acid peptide from snake venom; Syn-AKE is a synthetic dipeptide with similar activity
Argireline (Acetyl Hexapeptide-8)	Both studied for neuromuscular modulation	Argireline targets the SNARE complex; Syn-AKE targets nAChR at the postsynaptic membrane (different mechanism)
SNAP-8	Both are synthetic peptides for neuromodulation research	SNAP-8 inhibits SNARE complex assembly; Syn-AKE is an nAChR antagonist
Leuphasyl (Pentapeptide-18)	Both studied for neuromodulation	Leuphasyl is an enkephalin analog targeting opioid receptors; Syn-AKE targets nAChR
Botulinum Toxin	Both modulate neuromuscular transmission	Botulinum toxin enzymatically cleaves SNARE proteins with irreversible effects; Syn-AKE is a reversible nAChR antagonist

Research Applications

Researchers utilize Syn-AKE in a variety of laboratory research applications, primarily in the fields of neuropharmacology, receptor biology, and neuromuscular research. Key research applications include:

• Nicotinic Acetylcholine Receptor Studies: Investigating Syn-AKE’s binding affinity and antagonism of nAChR
• Neuromuscular Junction Research: Studying Syn-AKE’s effects on neuromuscular transmission and muscle contraction
• Synaptic Transmission Studies: Examining Syn-AKE’s modulation of neurotransmitter release and receptor activation
• Receptor-Ligand Interaction Research: Exploring the molecular basis of nAChR binding and antagonism
• Reversible Neuromuscular Blockade Studies: Preclinical research into reversible nAChR antagonism and receptor dynamics
• Biomimetic Peptide Research: Investigating synthetic mimics of natural venom peptides
• Muscle Contraction Modulation Studies: Studying Syn-AKE’s effects on contraction frequency and amplitude
• Receptor Binding Studies: Investigating the molecular interactions between Syn-AKE and nAChR subunits

Researchers who buy Syn-AKE in Switzerland can incorporate this peptide into their experimental protocols across these diverse research areas.

Syn-AKE Product Specifications

Specification	Detail
Product Name	Syn-AKE (Dipeptide Diaminobutyroyl Benzylamide Diacetate)
Amount	200mg
Form	Lyophilized powder
Sequence (One-Letter)	β-Ala-Pro-Dab-NHBzl
Sequence (Three-Letter)	beta-Ala-Pro-Dab-NH-benzyl
Molecular Formula	C23H37N5O7
Molecular Weight	Approximately 495.58 g/mol
CAS Number	823202-99-9
INCI Name	Dipeptide Diaminobutyroyl Benzylamide Diacetate
Synonyms	SYN®-AKE, Snake tripeptide, Syn-Ake acetate
Mechanism	Reversible antagonist of muscular nicotinic acetylcholine receptor (nAChR)
Research Category	Skin, Hair & Beauty Peptides
Purity	≥98% (HPLC)
Storage	Store desiccated at -20°C, protected from light

How to Evaluate Syn-AKE Quality

When sourcing Syn-AKE for research purposes, researchers should consider several quality indicators. A reliable Syn-AKE peptide supplier should provide:

• Identity Confirmation: Analytical verification that the peptide matches the expected sequence (β-Ala-Pro-Dab-NHBzl) and molecular weight (approximately 495.58 g/mol)
• Purity Analysis: Documentation of purity through methods such as HPLC – research grade typically provides ≥98% purity
• Batch Information: Clear lot numbers and batch traceability
• Certificate of Analysis (CoA): Documentation of analytical testing results
• Proper Labeling: Accurate product identification and storage instructions
• Appearance: White to off-white lyophilized powder

Researchers looking to buy Syn-AKE in Switzerland should seek suppliers who provide comprehensive documentation and quality assurance. Core Peptides remains committed to supporting research with properly documented products.

Understanding the Certificate of Analysis

A Certificate of Analysis (CoA) provides analytical data for a specific batch of a research peptide. For Syn-AKE research, a CoA typically includes:

• Identity Confirmation: Mass spectrometry confirming the peptide’s identity
• Purity Assessment: HPLC chromatograms showing the purity of the peptide
• Batch/Lot Information: Unique identifiers for traceability
• Analytical Methods: Description of the testing methods used

Importantly, researchers should understand that a CoA demonstrates the chemical identity and purity of a peptide but does not establish safety, efficacy, therapeutic effectiveness, or suitability for human use. The CoA serves as a quality control document for research purposes only.

Storage and Stability

Proper storage of research peptides is essential for maintaining their integrity and stability. For Syn-AKE, researchers should refer to the product documentation provided with their order for specific storage recommendations.

General best practices for peptide storage include keeping the lyophilized peptide desiccated, protected from light, and stored at -20°C or below. For specific temperature requirements and reconstitution protocols, consult the applicable product documentation or contact Core Peptides for the applicable storage specifications.

Research Considerations

Researchers incorporating Syn-AKE into their studies should be aware of several important considerations:

• Evidence Limitations: Syn-AKE has been studied in in vitro assays, receptor-binding studies, and clinical research. However, researchers should note that the peptide is supplied for laboratory research purposes only.
• Experimental Status: Researchers consider Syn-AKE a well-characterized compound, but findings should be interpreted within the context of ongoing research.
• Model Differences: Results from in vitro assays and cell culture models may not translate directly to all experimental outcomes.
• Regulatory Status: Syn-AKE is not FDA-approved and is intended for laboratory research use only.
• Quality Considerations: Researchers should verify the quality and identity of their Syn-AKE through appropriate analytical methods.
• Mechanistic Context: Syn-AKE’s proposed mechanisms involve nAChR antagonism and neuromuscular modulation. Researchers should consider appropriate experimental approaches to investigate these pathways.
• Reversibility: Syn-AKE acts as a reversible antagonist, which distinguishes it from irreversible neuromuscular blockers and may influence experimental design.

Researchers who buy Syn-AKE in Switzerland should ensure their studies include appropriate controls and interpret findings with scientific rigor.

Frequently Asked Questions About Syn-AKE

What is Syn-AKE?

Syn-AKE (Dipeptide Diaminobutyroyl Benzylamide Diacetate) is a synthetic dipeptide with the sequence β-Ala-Pro-Dab-NHBzl that mimics the neuromuscular blocking activity of Waglerin-1 from Temple Viper venom.

What is Syn-AKE researched for?

Researchers primarily investigate Syn-AKE for its reversible antagonism of muscular nicotinic acetylcholine receptors (nAChR), neuromuscular modulation, and synaptic transmission studies.

What is the amino acid sequence of Syn-AKE?

The amino acid sequence of Syn-AKE is β-Ala-Pro-Dab-NHBzl (beta-Ala-Pro-Dab-NH-benzyl).

What is the molecular weight of Syn-AKE?

The molecular weight of Syn-AKE is approximately 495.58 g/mol.

What is the CAS number for Syn-AKE?

The CAS number for Syn-AKE is 823202-99-9.

What is the mechanism of action for Syn-AKE?

Syn-AKE acts as a reversible antagonist of the muscular nicotinic acetylcholine receptor (mnAChR) at the postsynaptic membrane, inhibiting acetylcholine from binding to the receptor.

How does Syn-AKE differ from Waglerin-1?

Syn-AKE is a synthetic dipeptide that mimics the neuromuscular blocking activity of Waglerin-1, a 22-amino acid peptide found in Temple Viper venom, without the toxicity of the natural compound.

Can I buy Syn-AKE in Switzerland?

Yes, researchers can buy Syn-AKE in Switzerland from Core Peptides for laboratory research purposes.

What is the primary research category for Syn-AKE?

Syn-AKE belongs to the Skin, Hair & Beauty Peptides category, with secondary relevance to Cellular Research & Signaling research.

What related peptides are studied alongside Syn-AKE?

Related peptides include Argireline, SNAP-8, Leuphasyl, and other neuromuscular modulators.

What is the purity of Core Peptides’ Syn-AKE?

Core Peptides provides Syn-AKE with a purity of ≥98% as verified by HPLC analysis.

How should Syn-AKE be stored?

Syn-AKE should be stored lyophilized, desiccated, protected from light, at -20°C or below.

Is Syn-AKE soluble in water?

Yes, Syn-AKE is soluble in water and buffered aqueous solutions.

What is the difference between Syn-AKE and Argireline?

Syn-AKE targets nAChR at the postsynaptic membrane, while Argireline targets the SNARE complex. They have different mechanisms of action.

Related Research Peptides

Researchers interested in Syn-AKE may also find the following related peptides relevant to their work:

• Argireline (Acetyl Hexapeptide-8) – A SNARE-targeting peptide studied for neuromuscular modulation
• SNAP-8 – An extended analog of Argireline for SNARE complex research
• Leuphasyl (Pentapeptide-18) – An enkephalin analog studied for neuromodulation
• BPC-157 – A peptide studied for healing and tissue repair
• GHK-Cu – A copper peptide studied for cellular research

For a comprehensive selection of research peptides in Switzerland, explore the Core Peptides catalog.

Explore Skin, Hair & Beauty Peptides

Syn-AKE is part of our Skin, Hair & Beauty Peptides category, which features a range of research peptides investigated for their effects on cellular signaling, tissue biology, and neuromodulation. Browse our selection to find the right peptides for your laboratory research.

Explore Related Research Categories

Researchers may also find the following related categories relevant to Syn-AKE research:

• Cellular Research & Signaling – Peptides studied for receptor signaling and neuromuscular modulation
• Cognitive & Nootropic Peptides – Peptides studied for neurological and synaptic research
• Anti-Aging & Longevity – Peptides investigated for age-related research applications

Further Research

Researchers interested in learning more about Syn-AKE and related peptides should consult the primary scientific literature. Key areas of investigation include nAChR pharmacology, neuromuscular junction signaling, receptor-ligand interactions, and synaptic transmission. The PubMed database is an excellent resource for accessing peer-reviewed research articles on these topics.

Research Disclaimer

Core Peptides supplies Syn-AKE as a research peptide for laboratory research purposes only. All information provided is for educational and scientific reference purposes only and does not constitute medical advice. Researchers are responsible for ensuring compliance with all applicable laws and regulations governing the use of research peptides in their jurisdiction, including Switzerland.