Kisspeptin-10 (10MG)

What Is Kisspeptin-10 and Why Buy It for Research?

Buy Kisspeptin-10 in Switzerland for research into reproductive endocrinology, GPR54 signaling, and neuroendocrine regulation. Specifically, Kisspeptin-10 (also known as Kp-10, Metastin 45-54, or Kisspeptin-10 human) is a synthetic decapeptide composed of ten amino acids with the sequence H-Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂ (one-letter code: YNWNSFGLRF-NH₂). It is the shortest bioactive fragment of the larger kisspeptin-54 protein, which is encoded by the KISS1 gene first identified in 1996 at Pennsylvania State University.

Kisspeptin-10 has a molecular weight of 1302.4 g/mol and the molecular formula C₆₃H₈₃N₁₇O₁₄. The C-terminal amide group (-NH₂) on the final phenylalanine residue is a defining structural feature of the peptide, distinguishing it from truncated variants lacking this modification. In scientific research, Kisspeptin-10 serves as a molecular tool for studying GPR54 (KISS1R) signaling, HPG axis regulation, and reproductive endocrinology. Furthermore, Core Peptides supplies Kisspeptin-10 as a lyophilized powder exclusively for laboratory use. Therefore, researchers seeking to source this compound for their work will find it readily available when they buy Kisspeptin-10 in Switzerland.

Kisspeptin-10 Research Overview: Buy Kisspeptin-10 in Switzerland for GPR54 Studies

Centrally, research into Kisspeptin-10 has focused on its function as a potent endogenous agonist of the G protein-coupled receptor GPR54 (also known as KISS1R or AXOR12). Among the kisspeptin isoforms, Kp-10 is the smallest and is reported to activate KISS1R to its full functional potential. Kisspeptin-10 exhibits the same binding and agonist activity as the parent molecule, kisspeptin-54, and contains the basic residues required to retain full functional activity with higher receptor affinity and biopotency than longer forms of the peptide.

Kisspeptin-10 binds to the G protein-coupled receptor KISS1R/GPR54 with high affinity: Ki values of 1.59 nM for rat KISS1 receptors and 2.33 nM for human KISS1 receptors. This binding triggers G protein-coupled receptor signaling via activation of G(q) and phosphatidylinositol 4,5-bisphosphate hydrolysis. The activation of KISS1R by kisspeptin-10 recruits phosphatase DUSP18 and SRC to the KISS1R C-terminus. Measurement of calcium kinetics has revealed that kisspeptin-10 elicits a remarkable increase in intracellular calcium concentration in target cells.

Kisspeptins are reported to be the most potent activators of the hypothalamus-pituitary-gonadal (HPG) axis known to date. Kisspeptin potently elicits gonadotropin-releasing hormone (GnRH) release and luteinizing hormone (LH) secretion, even in the pre-pubertal period. Accordingly, researchers who buy Kisspeptin-10 in Switzerland from Core Peptides can support their investigations into GPR54 signaling, reproductive endocrinology, and neuroendocrine regulation.

How Does Kisspeptin-10 Work in Research?

Mechanistically, Kisspeptin-10’s proposed action involves functioning as a GPR54 agonist that activates Gq/11-coupled signaling to stimulate GnRH release and downstream gonadotropin secretion. The peptide operates through several interconnected mechanisms.

GPR54 Receptor Activation

Kisspeptin-10 binds to and activates the G protein-coupled receptor GPR54 (KISS1R), which is expressed on hypothalamic GnRH neurons and in other tissues. This binding event initiates a conformational change in the receptor, leading to the activation of downstream signaling pathways. Kisspeptin-10 exhibits higher receptor affinity and biopotency than longer forms of the peptide. The binding affinity (Ki) of Kisspeptin-10 for human KISS1R is approximately 2.33 nM.

Intracellular Signaling Cascade

The primary signaling pathway involves the coupling of activated KISS1R to the Gαq/11 subunit of the heterotrimeric G protein. This activation stimulates phospholipase C (PLC), which hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) into two key second messengers: inositol trisphosphate (IP3) and diacylglycerol (DAG). IP3 triggers the release of stored calcium (Ca²⁺) from the endoplasmic reticulum, and this sharp increase in intracellular calcium is critical for GnRH neuron depolarization and subsequent gonadotropin secretion.

GnRH and Gonadotropin Secretion

Kisspeptin-10 stimulates the release of gonadotropin-releasing hormone (GnRH) from hypothalamic neurons, which in turn drives the secretion of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) from the anterior pituitary. George et al. reported that kisspeptin-10 increased LH pulse frequency and LH secretion in healthy men, supporting its role in physiological GnRH pulse generation. Kisspeptin-10 significantly increased LH and testosterone concentrations, highlighting its potential therapeutic relevance in hypogonadal states. Studies have also demonstrated a central mechanism of action of kisspeptin-10 that is independent of sex steroids.

Osteoblast Differentiation

Beyond its neuroendocrine functions, Kisspeptin-10 has been shown to regulate osteoblast differentiation through GPR54-mediated activation of NFATc4 transcription factor. In mesenchymal stem cells, KP-10 enhances osteogenic gene expression, such as BMP2, Runx2, and Dlx5. This signaling pathway promotes Smad1/5/9 phosphorylation, a key step in BMP2-induced osteoblast differentiation. The mechanism involves NFATc4 binding to the BMP2 promoter, establishing Kisspeptin-10 as a regulator of bone formation through GPR54/NFATc4/BMP2 signaling.

Vasoconstriction and Angiogenesis Inhibition

Kisspeptin-10 is also a potent vasoconstrictor and inhibitor of angiogenesis. It acts as a tumor metastasis suppressor via its receptor GPR54. The ability of Kp-10 to inhibit signaling and chemotaxis induced by SDF-1 indicates that activation of GPR54 signaling may negatively regulate the role of CXCR4 in programming tumor metastasis.

Why Is Kisspeptin-10 Studied in Switzerland?

Primarily, researchers study Kisspeptin-10 because it represents the shortest bioactive kisspeptin isoform with full GPR54 agonist activity, enabling investigation of reproductive endocrinology, neuroendocrine regulation, and GPR54-mediated signaling pathways. In fact, this compound offers a focused tool for studying the molecular mechanisms underlying HPG axis function, fertility regulation, and gonadotropin secretion.

Research has demonstrated Kisspeptin-10’s relevance across multiple domains. Recent studies have revealed that the KISS1/KISS1R system plays an essential role in the neuroendocrine control of the reproductive axis. Development and functional analysis of Kp-10 analogs may help in the search for new agonists and antagonists as valuable tools to manipulate the KISS1/KISS1R system and hence fertility.

Furthermore, Kisspeptin-10 has been studied for its potential in treating stress-induced reproductive dysfunction. Kisspeptin-10 has also been studied for its antidepressant-like effects. Ultimately, researchers who buy Kisspeptin-10 in Switzerland gain access to a comprehensive research tool for investigating GPR54 signaling, reproductive endocrinology, and neuroendocrine regulation.

Kisspeptin-10 Research Areas in Laboratory Studies

Research Area	Focus	Evidence Level
GPR54 Receptor Pharmacology	Receptor binding, Gq/11 signaling, calcium mobilization	Mechanistic / In Vitro
HPG Axis Regulation	GnRH release, LH secretion, FSH regulation, puberty onset	Mechanistic / In Vivo / Clinical
Fertility Research	Ovulation induction, gametogenesis, reproductive function	Preclinical / Clinical
Neuroendocrine Research	Hypothalamic-pituitary signaling, stress-induced dysfunction	Preclinical
Osteoblast Differentiation	BMP2 expression, NFATc4 activation, bone formation	Mechanistic / In Vitro
Vasoconstriction and Angiogenesis	Vascular tone regulation, angiogenesis inhibition	Mechanistic / Preclinical
Metastasis Research	Tumor suppression, CXCR4 regulation, migration inhibition	Mechanistic / Preclinical
Metabolic Regulation	Obesity-diabetes, insulin secretion, energy metabolism	Preclinical

Kisspeptin-10 Compared With Related Research Compounds

Compound	Research Focus	Mechanism / Target	Key Difference
Kisspeptin-10 (Kp-10)	GPR54 signaling, HPG axis, fertility, osteogenesis	KISS1R/GPR54 agonist (Gq/11 signaling)	Shortest bioactive kisspeptin; full GPR54 agonist activity
Kisspeptin-54 (Metastin)	GPR54 signaling, metastasis suppression	KISS1R/GPR54 agonist	Longer isoform; proteolytically cleaved to generate Kp-10
Kisspeptin-234 (Antagonist)	GPR54 antagonism, receptor blockade	KISS1R antagonist	Receptor antagonist; opposite mechanism
Gonadorelin (GnRH)	GnRH receptor activation, gonadotropin secretion	GnRH receptor agonist	Acts downstream of kisspeptin; different receptor target

Comparatively, Kisspeptin-10 differs from other kisspeptin isoforms in its length and research applications. On the one hand, Kisspeptin-10 is the shortest isoform (10 amino acids) that retains full GPR54 agonist activity and exhibits higher receptor affinity than longer forms. On the other hand, Kisspeptin-54 is the full-length protein that is proteolytically cleaved to generate Kp-10 and other isoforms.

Research Applications for Kisspeptin-10 in Switzerland

Scientists have investigated Kisspeptin-10 across several research domains:

• GPR54 Receptor Pharmacology: Researchers use Kisspeptin-10 to study receptor binding, Gq/11 signaling, calcium mobilization, and KISS1R-mediated pathways.
• Reproductive Endocrinology: The peptide is employed to investigate HPG axis regulation, GnRH release, LH and FSH secretion, and fertility mechanisms.
• Fertility Research: Kisspeptin-10 has been studied for ovulation induction, gametogenesis, and reproductive function in preclinical and clinical models.
• Neuroendocrine Research: Studies have examined Kisspeptin-10’s role in stress-induced reproductive dysfunction and neuroendocrine signaling.
• Osteogenesis Research: The peptide has been investigated for its effects on BMP2 expression, NFATc4 activation, and osteoblast differentiation.
• Vascular Research: Kisspeptin-10 has been studied for its vasoconstrictor properties and angiogenesis inhibition.

Evidently, researchers who buy Kisspeptin-10 in Switzerland gain a valuable addition to any laboratory toolkit focused on reproductive endocrinology, GPR54 signaling, and neuroendocrine research.

Kisspeptin-10 Product Specifications

Specification	Details
Product Name	Kisspeptin-10 (Kp-10)
Alternative Names	Metastin (45-54), Kisspeptin-10 human, Kp-10, Kisspeptin decapeptide
Amount	1mg
Form	Lyophilized powder
Purity	Not verified (≥95-98% HPLC purity reported by some suppliers)
Amino Acid Sequence	H-Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂
Sequence (1-letter)	YNWNSFGLRF-NH₂
Peptide Length	10 amino acids (decapeptide)
Molecular Formula	C₆₃H₈₃N₁₇O₁₄
Molecular Weight	1302.4 g/mol
CAS Number	374675-21-5
PubChem CID	25240297
Receptor Binding Affinity (Ki)	2.33 nM (human KISS1R)
Research Category	Fertility & Hormone Research / Sexual Health
Research Use	Laboratory research use only
CoA	Available upon request (not verified)
Storage	Lyophilized powder should be stored desiccated at -20°C or below, protected from light
Solubility	Soluble in water and bacteriostatic water

How to Evaluate Kisspeptin-10 Quality for Research

When sourcing Kisspeptin-10 peptide for laboratory research, researchers should consider several quality indicators:

• Identity Confirmation: Analytical techniques such as mass spectrometry should confirm the compound’s molecular weight (1302.4 g/mol) and sequence (H-Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂).
• Purity Assessment: High-performance liquid chromatography (HPLC) should verify purity, with ≥95% considered standard for research-grade material.
• Batch Traceability: Lot numbers and batch information enable researchers to track specific production runs.
• Certificate of Analysis: A CoA should accompany the product, detailing analytical testing results including HPLC purity and identity confirmation.
• Labeling Accuracy: Proper labeling should clearly indicate the product name, amount (1mg), sequence, molecular formula, CAS number, and storage recommendations.
• Storage Conditions: Appropriate storage at -20°C or below in desiccated, dark conditions helps maintain peptide stability.

Understanding the Certificate of Analysis

Typically, a Certificate of Analysis (CoA) accompanies research peptides and provides information about the specific batch’s analytical testing. For example, a CoA may include product identity confirmation, purity percentage as determined by HPLC, batch or lot number for traceability, analytical methods used (e.g., mass spectrometry, HPLC), and a physical description of the product.

Nevertheless, it is important to note that a CoA does not establish safety, efficacy, therapeutic effectiveness, or suitability for human use. Instead, it serves as a quality control document that helps researchers verify the identity and purity of the peptide they are using in their laboratory investigations.

Storage and Stability of Kisspeptin-10

Core Peptides supplies Kisspeptin-10 as a lyophilized (freeze-dried) powder. To maintain stability and integrity:

• Store the lyophilized powder desiccated at -20°C or below for long-term storage
• Store at 4°C for short-term storage if frequent access is required
• Avoid repeated freeze-thaw cycles
• Weigh under dry conditions; the peptide is hygroscopic
• Reconstitute only immediately before use with appropriate sterile solvents (sterile water or bacteriostatic water)
• Inject solvent slowly against the inner wall of the vial; avoid vigorous agitation during dissolution
• Filter sterilize if required (e.g., 0.22 µm) immediately prior to use
• Aliquot and store reconstituted solutions at -20°C or below to minimize degradation
• Protect from light and moisture; prolonged exposure to light, elevated temperature, or aqueous environments may reduce stability

Frequently Asked Questions About Buying Kisspeptin-10 in Switzerland

What is Kisspeptin-10?

Specifically, Kisspeptin-10 (Kp-10, Metastin 45-54) is a synthetic decapeptide with the sequence H-Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂ (YNWNSFGLRF-NH₂). It is the shortest bioactive fragment of the kisspeptin-54 protein and a potent endogenous agonist of the GPR54 (KISS1R) receptor.

What is Kisspeptin-10 researched for?

Primarily, researchers study Kisspeptin-10 for its role as a GPR54 agonist in reproductive endocrinology, HPG axis regulation, and neuroendocrine signaling. Studies have investigated its effects on GnRH and LH secretion, fertility, osteoblast differentiation, and vasoconstriction.

How does Kisspeptin-10 work?

Mechanistically, Kisspeptin-10 binds to and activates GPR54 (KISS1R), a GPCR on hypothalamic GnRH neurons, triggering Gq/11 signaling, PLC activation, calcium mobilization, and GnRH release, which in turn drives LH and FSH secretion from the pituitary.

What is the amino acid sequence of Kisspeptin-10?

The amino acid sequence is H-Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂ (one-letter code: YNWNSFGLRF-NH₂).

What is the molecular weight of Kisspeptin-10?

The molecular weight is 1302.4 g/mol.

What is the molecular formula of Kisspeptin-10?

The molecular formula is C₆₃H₈₃N₁₇O₁₄.

What is the CAS number of Kisspeptin-10?

The CAS number is 374675-21-5.

Is Kisspeptin-10 synthetic?

Yes, Kisspeptin-10 is a synthetic peptide produced through solid-phase peptide synthesis.

What is the binding affinity of Kisspeptin-10 for GPR54?

Kisspeptin-10 binds to human KISS1R with a Ki of 2.33 nM and to rat KISS1R with a Ki of 1.59 nM.

How does Kisspeptin-10 differ from kisspeptin-54?

Kisspeptin-10 is the shortest bioactive isoform (10 amino acids) derived from the larger kisspeptin-54 protein. Kp-10 exhibits the same binding and agonist activity as the parent molecule with higher receptor affinity and biopotency.

What research exists on Kisspeptin-10?

Research on Kisspeptin-10 includes studies demonstrating its role as the most potent activator of the HPG axis, its effects on LH secretion and pulse frequency in healthy men, its role in osteoblast differentiation via BMP2/NFATc4 signaling, and its vasoconstrictor and angiogenesis inhibitory properties.

Where can I buy Kisspeptin-10 in Switzerland?

You can buy Kisspeptin-10 in Switzerland from Core Peptides, a trusted research peptide supplier serving the Swiss scientific community.

How should researchers evaluate Kisspeptin-10 quality?

Researchers should look for identity confirmation via mass spectrometry, purity data from HPLC, batch traceability, and a Certificate of Analysis.

Related Research Compounds

Researchers interested in Kisspeptin-10 may also find the following related compounds relevant to their work:

• Kisspeptin-54 — The full-length kisspeptin protein from which Kp-10 is derived, researched for GPR54 signaling and metastasis suppression.
• Kisspeptin-234 — A kisspeptin receptor antagonist researched for receptor blockade and HPG axis inhibition studies.
• Gonadorelin (GnRH) — The hypothalamic decapeptide that acts downstream of kisspeptin, researched for gonadotropin secretion and fertility.
• GHRP-2 — A growth hormone secretagogue researched for GH release and diagnostic applications.

Explore Fertility & Hormone Research

Kisspeptin-10 belongs to the Fertility & Hormone Research category of research compounds. Fertility & Hormone Research products are studied for their potential roles in reproductive endocrinology, hormone regulation, and fertility research. Generally, researchers who buy Kisspeptin-10 in Switzerland often pair it with related compounds in this category to explore complementary pathways in reproductive research and endocrine signaling.

Explore Sexual Health

Researchers studying reproductive function, hormonal regulation, and sexual health may also find relevant compounds in the Sexual Health category. Kisspeptin-10’s role in the HPG axis and gonadotropin regulation makes it a valuable tool for investigating reproductive and sexual health research.

Further Research

For researchers seeking to deepen their understanding of Kisspeptin-10 and its research applications, the following areas may warrant further investigation:

• The relationship between Kisspeptin-10-mediated GPR54 activation and specific downstream signaling pathways in different cell types
• Comparative studies between Kisspeptin-10 and longer kisspeptin isoforms in various reproductive and neuroendocrine models
• The potential for Kisspeptin-10 to provide insights into stress-induced reproductive dysfunction and fertility regulation
• Mechanistic studies on Kisspeptin-10’s interaction with GPR54 and its functional consequences for GnRH secretion
• Exploration of Kisspeptin-10’s effects on different tissue systems beyond the reproductive axis

Research Disclaimer

Researchers must not use it for human consumption, injection, or any form of therapeutic application. Moreover, this product is not a medicine, pharmaceutical, or medical device. Furthermore, Swissmedic, the FDA, EMA, or any other regulatory authority have not approved it for human use. Researchers should consult Swissmedic for applicable regulations and guidance on research peptide handling in Switzerland.

Generally, the information provided on this page is for educational and scientific reference purposes only. Therefore, it does not constitute medical advice, treatment recommendations, or dosing instructions. Ultimately, researchers bear the responsibility for ensuring that their use of this product complies with all applicable laws, regulations, and institutional guidelines.